Recombinant humaan DERA-eiwit

DERA, also known as deoxyribose-phosphate aldolase, belongs to the deoC/fbaB aldolase protein family involved in the carbohydrate degradation pathway. This protein catalyzes the conversion of 2-deoxy-D-ribose 5-phosphate to D-glyceraldehyde 3-phosphate and an acetyldehyde. Recombinant human DERA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Putative deoxyribose-phosphate aldolase, CGI-26, DEOC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.3 kDa (338aa) confirmed by MALDI-TOF

DERA, Putative deoxyribose-phosphate aldolase, CGI-26, DEOC, ATGP1196-10 µg, ATGP1196-20 µg, ATGP1196-50 µg, ATGP1196-100 µg, ATGP1196-250 µg, ATGP1196-500 µg, ATGP1196-1 mg, ATGP1196-10, ATGP1196-20, ATGP1196-50, ATGP1196-100, ATGP1196-250, ATGP1196-500, ATGP1196-1

Heine, A. et al. (2004) J. Mol. Biol. 343:1019-34.; ; Sakuraba, H. et al. (2003) J. Biol. Chem. 278:10799-806

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Metabolism

NP_057038

Q9Y315

Human

MSAHNRGTEL DLSWISKIQV NHPAVLRRAE QIQARRTVKK EWQAAWLLKA VTFIDLTTLS GDDTSSNIQR LCYKAKYPIR EDLLKALNMH DKGITTAAVC VYPARVCDAV KALKAAGCNI PVASVAAGFP AGQTHLKTRL EEIRLAVEDG ATEIDVVINR SLVLTGQWEA LYDEIRQFRK ACGEAHLKTI LATGELGTLT NVYKASMIAM MAGSDFIKTS TGKETVNATF PVAIVMLRAI RDFFWKTGNK IGFKPAGGIR SAKDSLAWLS LVKEELGDEW LKPELFRIGA STLLSDIERQ IYHHVTGRYA AYHDLPMS