Recombinant humaan desert egel/dh (C23II) eiwit

DHH is a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. Recombinant human DHH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Desert hedgehog protein, desert hedgehog, GDXYM, HHG-3, SRXY7

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

22.4 kDa (201aa) confirmed by MALDI-TOF

Desert Hedgehog, Dhh (C23II), Desert hedgehog protein, desert hedgehog, GDXYM, HHG-3, SRXY7, ATGP2221-10 µg, ATGP2221-20 µg, ATGP2221-50 µg, ATGP2221-100 µg, ATGP2221-250 µg, ATGP2221-500 µg, ATGP2221-1 mg, ATGP2221-10, ATGP2221-20, ATGP2221-50, ATGP2221-100, ATGP2221-250, ATGP2221-500, ATGP2221-1

Chen YJ, et al. (2007) . Cell Cycle. 6 (15) :1826-30.; ; Van den Brink GR., et al. (2007) . Physiol Rev. 87 (4) :1343-75

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Stem cell

NP_066382

O43323

Human

, MGSSHHHHHH SSGLVPRGSH MGSM>IIGPGR GPVGRRRYAR KQLVPLLYKQ FVPGVPERTL GASGPAEGRV ARGSERFRDL VPNYNPDIIF KDEENSGADR LMTERCKERV NALAIAVMNM WPGVRLRVTE GWDEDGHHAQ DSLHYEGRAL DITTSDRDRN KYGLLARLAV EAGFDWVYYE SRNHVHVSVK ADNSLAVRAG G