Recombinant humaan DESI1-eiwit

DESI1 belongs to the DeSI family and contains 1 PPPDE peptidase domain. This protein is a protease which deconjugates SuMO1, SuMO2 and SuMO3 from some substrate proteins and has isopeptidase but not SuMO-processing activity. DESI1 desumoylates ZBTB46. Recombinant human DESI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Desumoylating isopeptidase 1, D15Wsu75e, DeSI-1, DESI2, DJ347H13.4, FAM152B, PPPDE2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.7 kDa (191aa) confirmed by MALDI-TOF

DESI1, Desumoylating isopeptidase 1, D15Wsu75e, DeSI-1, DESI2, DJ347H13.4, FAM152B, PPPDE2, ATGP2242-10 µg, ATGP2242-20 µg, ATGP2242-50 µg, ATGP2242-100 µg, ATGP2242-250 µg, ATGP2242-500 µg, ATGP2242-1 mg, ATGP2242-10, ATGP2242-20, ATGP2242-50, ATGP2242-100, ATGP2242-250, ATGP2242-500, ATGP2242-1

Briegel W., et al. (2004) Psychother. 32:107-115; ; Gothelf D., et al. (2008) Dev Disabil res Rev. 14:59-68

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_056519

Q6ICB0

Human

MGSSHHHHHH SSGLVPRGSH MGSMEPPNLY PVKLYVYDLS KGLARRLSPI MLGKQLEGIW HTSIVVHKDE FFFGSGGISS CPPGGTLLGP PDSVVDVGST EVTEEIFLEY LSSLGESLFR GEAYNLFEHN CNTFSNEVAQ FLTGRKIPSY ITDLPSEVLS TPFGQALRPL LDSIQIQPPG GSSVGRPNGQ S