Recombinant humaan DHODH-eiwit

DHODH, also known as dihydroorotate dehydrogenase (quinone), mitochondrial, belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Recombinant human DHODH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dihydroorotate dehydrogenase (quinone) mitochondrial, Dihydroorotate dehydrogenase (quinone), mitochondrial, DHOdehase, POADS, uRA1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.3 kDa (390aa) confirmed by MALDI-TOF

DHODH, Dihydroorotate dehydrogenase (quinone) mitochondrial, Dihydroorotate dehydrogenase (quinone), mitochondrial, DHOdehase, POADS, uRA1, ATGP1615-10 µg, ATGP1615-20 µg, ATGP1615-50 µg, ATGP1615-100 µg, ATGP1615-250 µg, ATGP1615-500 µg, ATGP1615-1 mg, ATGP1615-10, ATGP1615-20, ATGP1615-50, ATGP1615-100, ATGP1615-250, ATGP1615-500, ATGP1615-1

Rawls J., et al. (2000) Eur. J. Biochem. 267:2079-2087; ; Liu S., et al. (2000) Structure. 8:25-33

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Metabolism

NP_001352

Q02127

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR