Recombinant humaan DHPS-eiwit

DHPS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. This protein catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. Recombinant DHPS protein was expressed in E. coli and purified by using conventional chromatography techniques.

Deoxyhypusine synthase, MIG13, Deoxyhypusine synthase DHS, DS, Migration inducing gene 13.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

43.1 kDa (389aa) confirmed by MALDI-TOF

DHPS, Deoxyhypusine synthase, MIG13, Deoxyhypusine synthase DHS, DS, Migration inducing gene 13., ATGP0500-10 µg, ATGP0500-20 µg, ATGP0500-50 µg, ATGP0500-100 µg, ATGP0500-250 µg, ATGP0500-500 µg, ATGP0500-1 mg, ATGP0500-10, ATGP0500-20, ATGP0500-50, ATGP0500-100, ATGP0500-250, ATGP0500-500, ATGP0500-1

Umland TC, et al. (2004) J. Biol. Chem. 279 (27) : 28697-705; ; Jones T, et al. (1996) Genomics. 35 (3) : 635-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_001921.1

P49366

Human

MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNED