Recombinant humaan DHRS4-eiwit

DHRS4, also known as dehydrogenase/reductase SDR family member 4, belongs to the short-chain dehydrogenases/reductases (SDR) family. DHRS4 reduces all trans retinal and 9 cis retinal. This protein can also catalyze the oxidation of all trans retinol with NADP as cofactor, but with much lower efficiency. Also, DHRS4 reduces alkyl phenyl ketones and alpha dicarbonyl compounds with aromatic rings, such as pyrimidine 4 aldehyde, 3 benzoylpyridine, 4 benzoylpyridine, menadione and 4 hexanoylpyridine. Recombinant human DHRS4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dehydrogenase/reductase SDR family member 4, CR, NRDR, PHCR, PSCD, SCAD-SRL, SDR-SRL, SDR25C1, SDR25C2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.1 kDa (302aa) confirmed by MALDI-TOF

DHRS4, Dehydrogenase/reductase SDR family member 4, CR, NRDR, PHCR, PSCD, SCAD-SRL, SDR-SRL, SDR25C1, SDR25C2, ATGP1326-10 µg, ATGP1326-20 µg, ATGP1326-50 µg, ATGP1326-100 µg, ATGP1326-250 µg, ATGP1326-500 µg, ATGP1326-1 mg, ATGP1326-10, ATGP1326-20, ATGP1326-50, ATGP1326-100, ATGP1326-250, ATGP1326-500, ATGP1326-1

Fransen M., et al. (1999) Biochem. J. 340:561-568; ; Du J., et al. (2004) Yi Chuan Xue Bao. 31:661-667

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 1mM DTT

Redox Proteins

NP_066284

Q9BTZ2

Human

MHKAGL LGLCARAWNS VRMASSGMTR RDPLANKVAL VTASTDGIGF AIARRLAQDG AHVVVSSRKQ QNVDQAVATL QGEGLSVTGT VCHVGKAEDR ERLVATAVKL HGGIDILVSN AAVNPFFGSI MDVTEEVWDK TLDINVKAPA LMTKAVVPEM EKRGGGSVVI VSSIAAFSPS PGFSPYNVSK TALLGLTKTL AIELAPRNIR VNCLAPGLIK TSFSRMLWMD KEKEESMKET LRIRRLGEPE DCAGIVSFLC SEDASYITGE TVVVGGGTPS RL