Recombinant humaan DHRS9-eiwit

DHRS9, also known as dehydrogenase/reductase SDR family member 9, functions as a homotetramer that converts both 3-alpha-tetrahydroprogesterone (allopregnanolone) and 3-alpha-androstanediol to dihydroxyprogesterone and is thought to play a role in retinoic acid biosynthesis. Recombinant human DHRS9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, 3-alpha-HSD, 3ALPHA-HSD, 3alpha-HSD, Dehydrogenase/reductase SDR family member 9

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.9 kDa (327aa) confirmed by MALDI-TOF

DHRS9, Dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, 3-alpha-HSD, 3ALPHA-HSD, 3alpha-HSD, Dehydrogenase/reductase SDR family member 9, ATGP1343-10 µg, ATGP1343-20 µg, ATGP1343-50 µg, ATGP1343-100 µg, ATGP1343-250 µg, ATGP1343-500 µg, ATGP1343-1 mg, ATGP1343-10, ATGP1343-20, ATGP1343-50, ATGP1343-100, ATGP1343-250, ATGP1343-500, ATGP1343-1

Chetyrkin S.V. et al. (2001) J. Biol.Chem. 276: 22278-22286.; ; Jones R.J. et al. (2007) . J. Biol.Chem. 282: 8317-8324.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Metabolism

NP_005762

Q9BPW9

Human

MGSSHHHHHH SSGLVPRGSH MGSHMRKGKL KIEDITDKYI FITGCDSGFG NLAARTFDKK GFHVIAACLT ESGSTALKAE TSERLRTVLL DVTDPENVKR TAQWVKNQVG EKGLWGLINN AGVPGVLAPT DWLTLEDYRE PIEVNLFGLI SVTLNMLPLV KKAQGRVINV SSVGGRLAIV GGGYTPSKYA VEGFNDSLRR DMKAFGVHVS CIEPGLFKTN LADPVKVIEK KLAIWEQLSP DIKQQYGEGY IEKSLDKLKG NKSYVNMDLS PVVECMDHAL TSLFPKTHYA AGKDAKIFWI PLSHMPAALQ DFLLLKQKAE LANPKAV