Recombinant humaan dihydrofolaat Reductase/DHFR-eiwit

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Dihydrofolate reductase, EC 1.5.1.3

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

23.6 kDa (207aa) confirmed by MALDI-TOF

Dihydrofolate Reductase, DHFR, ATGP0371-10 µg, ATGP0371-20 µg, ATGP0371-50 µg, ATGP0371-100 µg, ATGP0371-250 µg, ATGP0371-500 µg, ATGP0371-1 mg, ATGP0371-10, ATGP0371-20, ATGP0371-50, ATGP0371-100, ATGP0371-250, ATGP0371-500, ATGP0371-1

Loveridge EJ., et al. (2009) Biochemistry. 48 (25) :5922-33. ; ; Hu Y., et al. (2009) J Cell Biol. 185 (1) :87-100.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

Redox Proteins

NP_000782.1

P00374

Human

MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND