Recombinant humaan DIMT1-eiwit

DIMT1 (DIM1 dimethyladenosine transferase 1 homolog) belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus. Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DIM1 dimethyladenosine transferase 1 homolog, DIM1, DIMT1L, HSA9761, HuSSY5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.5 kDa (334aa) confirmed by MALDI-TOF

DIMT1, DIM1 dimethyladenosine transferase 1 homolog, DIM1, DIMT1L, HSA9761, HuSSY5, ATGP1622-10 µg, ATGP1622-20 µg, ATGP1622-50 µg, ATGP1622-100 µg, ATGP1622-250 µg, ATGP1622-500 µg, ATGP1622-1 mg, ATGP1622-10, ATGP1622-20, ATGP1622-50, ATGP1622-100, ATGP1622-250, ATGP1622-500, ATGP1622-1

Scherl A., et al. (2002) Mol. Biol. Cell 13:4100-4109; ; Saltman, et al. (1993) Genomics 16:726-732.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200 mM NaCl, 2mM EDTA

Epigenomics (Transcription & Translation)

NP_055288

Q9UNQ2

Human

MPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS