Recombinant humaan DKK3-eiwit

Dickkopf-related protein 3, also known as DKK3, is a member of the DKK protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Recombinant human DKK3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, REIC, RIG, CRRL

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.8 kDa (353aa)

Cardiomyocyte regeneration-related lncRNA, CRRL, Dickkopf, Dickkopf 3 homolog, Dickkopf WNT signaling pathway inhibitor 3, Dickkopf-3, Dickkopf-related protein 3, Dickkopf-related protein 3 precursor, DKK3, Dkk-3, Regulated in glioma, REIC, RIG, Xenopus laevis, Xenopus laevis homolog 3, ATGP1666-10 µg, ATGP1666-20 µg, ATGP1666-50 µg, ATGP1666-100 µg, ATGP1666-250 µg, ATGP1666-500 µg, ATGP1666-1 mg, ATGP1666-010, ATGP1666-020, ATGP1666-050, ATGP1666-100, ATGP1666-250, ATGP1666-500, ATGP1666-01M

Cadigan K M., et al. (1997) Genes Dev. 11:3286-3305.; ; Gilnka A., et al. (1998) Nature. 391:357-362.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea,10% glycerol, 1mM DTT

Signal Transduction

NP_001018067.1

Q9UBP4

Human

APAPTA TSAPVKPGPA LSYPQEEATL NEMFREVEEL MEDTQHKLRS AVEEMEAEEA AAKASSEVNL ANLPPSYHNE TNTDTKVGNN TIHVHREIHK ITNNQTGQMV FSETVITSVG DEEGRRSHEC IIDEDCGPSM YCQFASFQYT CQPCRGQRML CTRDSECCGD QLCVWGHCTK MATRGSNGTI CDNQRDCQPG LCCAFQRGLL FPVCTPLPVE GELCHDPASR LLDLITWELE PDGALDRCPC ASGLLCQPHS HSLVYVCKPT FVGSRDQDGE ILLPREVPDE YEVGSFMEEV RQELEDLERS LTEEMALREP AAAAAALLGG EEI