Recombinant humaan DLL4-eiwit

DLL4, also known as Delta-Like protein 4, is a membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. It is predicted to encode a membrane-bound ligand, characterized by an extracellular region containing several EGF-like domains and a DSL domain required for receptor binding. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. The Notch signaling pathway is fundamental to proper cardiovascular development and is now recognized as an important player in tumor angiogenesis. Two key Notch ligands have been implicated in tumor angiogenesis, Delta-like 4 and Jagged1. Recombinant human DLL4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Delta-like protein 4, Drosophila Delta homolog 4, Delta4, delta like canonical Notch ligand 4, AOS6, hdelta2

HEK293

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

55.1kDa (504aa)

, ATGP3954-10 µg, ATGP3954-20 µg, ATGP3954-50 µg, ATGP3954-100 µg, ATGP3954-250 µg, ATGP3954-500 µg, ATGP3954-1 mg, ATGP3954-10, ATGP3954-20, ATGP3954-50, ATGP3954-100, ATGP3954-250, ATGP3954-500, ATGP3954-1

J R Shutter., et al, (2000) Genes Dev. 14:1313-1318.; ; J Dufraine., et al, (2008) Oncogene. 27:5132-5137.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_061947

Q9NR61

Human

Ser