Recombinant humaan DNA polymerase beta/POLB-eiwit

DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA polymerase beta, DNA directed DNA polymerase beta, Polymerase (DNA directed) beta, DNA pol beta, DNA polymerase beta subunit, MGC125976, Pol B, Pol beta

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

40.3 kDa (355aa) confirmed by MALDI-TOF

POLB, DNA polymerase beta, DNA directed DNA polymerase beta, DNA pol beta, DNA polymerase beta, DNA polymerase beta subunit, MGC125976, Pol B, Pol beta, PolB, Polymerase (DNA directed) beta, ATGP1190-10 µg, ATGP1190-20 µg, ATGP1190-50 µg, ATGP1190-100 µg, ATGP1190-250 µg, ATGP1190-500 µg, ATGP1190-1 mg, ATGP1190-10, ATGP1190-20, ATGP1190-50, ATGP1190-100, ATGP1190-250, ATGP1190-500, ATGP1190-1

Bambara R A., et al. (1991) Biochim Biophys Acta. 1088:11-24.; ; Ropp P A., et al. (1996) Genomics. 36:449-458.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_002681.1

P06746

Human

MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE