Recombinant humaan DNA polymerase delta subeenheid 4/POLD4-eiwit

POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human POLD4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA polymerase delta 4 accessory subunit, DNA polymerase delta smallest subunit p12, DNA polymerase delta subunit p12

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.8 kDa (130aa) confirmed by MALDI-TOF

POLD4, DNA polymerase delta subunit 4, p12, POLDS, ATGP2282-10 µg, ATGP2282-20 µg, ATGP2282-50 µg, ATGP2282-100 µg, ATGP2282-250 µg, ATGP2282-500 µg, ATGP2282-1 mg, ATGP2282-10, ATGP2282-20, ATGP2282-50, ATGP2282-100, ATGP2282-250, ATGP2282-500, ATGP2282-1

Taylor T.D., et al. (2006) Nature. 440:497-500; ; Gerhard D.S., et al. (2004) Genome Res. 14:2121-2127

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_066996

Q9HCU8

Human

MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL