Recombinant humaan DNA polymerase lambda/POLL-eiwit

DNA polymerase lambda, also known as POLL, is a DNA polymerase involved in base excision repair (BER) . POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA polymerase lambda, BETA-N, POL-KAPPA, Pol beta2, POLKAPPA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36 kDa (320aa) confirmed by MALDI-TOF

POLL, ATGP1255-10 µg, ATGP1255-20 µg, ATGP1255-50 µg, ATGP1255-100 µg, ATGP1255-250 µg, ATGP1255-500 µg, ATGP1255-1 mg, ATGP1255-10, ATGP1255-20, ATGP1255-50, ATGP1255-100, ATGP1255-250, ATGP1255-500, ATGP1255-1

O Wang J., et al. (2001) Cancer Res. 61:5366-5369.; ; Zhang Y., et al. (2000) Nucleic Acids Res. 28:4147-4156.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Epigenetics and Nuclear Signaling

AAH03548

Q9UGP5

Human

MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW