Recombinant humaan DNAJB2-eiwit

DnaJB2, also known as HSJ1 or HSPF3, is belongs to the DnaJ family. It is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The DnaJ proteins play a critical role in the HSP70 chaperone machine by interacting with HSP70 to stimulate ATP hydrolysis. DnaJB2 is important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. Recombinant human DNAJB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DnaJ homolog subfamily B member 2, HSJ1, HSPF3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33 kDa (300aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

DNAJB2, DnaJ homolog subfamily B member 2, HSJ1, HSPF3, ATGP0788-10 µg, ATGP0788-20 µg, ATGP0788-50 µg, ATGP0788-100 µg, ATGP0788-250 µg, ATGP0788-500 µg, ATGP0788-1 mg, ATGP0788-10, ATGP0788-20, ATGP0788-50, ATGP0788-100, ATGP0788-250, ATGP0788-500, ATGP0788-1

Stewart GR., et al. (2004) Tuberculosis. 84 (3-4) :180-7. ; ; Tomoyasu T., et al. (1998) Mol Microbiol. 30 (3) :567-81.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl

Heat Shock Proteins

NP_001034639

P25686

Human

MGSSHHHHHH SSGLVPRGSH MGSMASYYEI LDVPRSASAD DIKKAYRRKA LQWHPDKNPD NKEFAEKKFK EVAEAYEVLS DKHKREIYDR YGREGLTGTG TGPSRAEAGS GGPGFTFTFR SPEEVFREFF GSGDPFAELF DDLGPFSELQ NRGSRHSGPF FTFSSSFPGH SDFSSSSFSF SPGAGAFRSV STSTTFVQGR RITTRRIMEN GQERVEVEED GQLKSVTING VPDDLALGLE LSRREQQPSV TSRSGGTQVQ QTPASCPLDS DLSEDEDLQL AMAYSLSEME AAGKKPADVF