Recombinant humaan DNAJB8-eiwit

DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de) acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation. Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

DnaJ homolog subfamily B member 8

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.8 kDa (252aa) confirmed by MALDI-TOF

DNAJB8, DnaJ homolog subfamily B member 8, ATGP1265-10 µg, ATGP1265-20 µg, ATGP1265-50 µg, ATGP1265-100 µg, ATGP1265-250 µg, ATGP1265-500 µg, ATGP1265-1 mg, ATGP1265-10, ATGP1265-20, ATGP1265-50, ATGP1265-100, ATGP1265-250, ATGP1265-500, ATGP1265-1

Hageman J., et al. (2010) Mol Cell. 37 (3) :355-69.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Heat Shock Proteins

NP_699161

Q8NHS0

Human

MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK