Recombinant humaan DNAJC12-eiwit

DNAJC12, also known as JDP1, is a member of the DnaJ/Hsp40 family. The members of the DnaJ/Hsp40 proteins are highly conserved through evolution, expressed in several tissues and act as co-chaperone regulating protein folding, transport, translational initiation and gene expression. DnaJC12 may play a role in protein folding and export and is thought to be involved in estrogen control and the development of breast cancer. This protein is expressed at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. Recombinant human DNAJC12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DnaJ (Hsp40) homolog subfamily C member 12, DnaJ (Hsp40) homolog, subfamily C, member 12, JDP1, RP11-57G10.2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26kDa (222aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

DNAJC12, DnaJ (Hsp40) homolog subfamily C member 12, DnaJ (Hsp40) homolog, subfamily C, member 12, JDP1, RP11-57G10.2, ATGP1636-10 µg, ATGP1636-20 µg, ATGP1636-50 µg, ATGP1636-100 µg, ATGP1636-250 µg, ATGP1636-500 µg, ATGP1636-1 mg, ATGP1636-10, ATGP1636-20, ATGP1636-50, ATGP1636-100, ATGP1636-250, ATGP1636-500, ATGP1636-1

De Bessa SA, et al. (2006) J Mol Med. 17 (2) :363-7.; ; Mahlknecht, u., et al. (2009) Int. J. Mol. Med. 23: 245-252.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Heat Shock Proteins

NP_068572

Q9UKB3

Human

MGSSHHHHHH SSGLVPRGSH MGSHMDAILN YRSEDTEDYY TLLGCDELSS VEQILAEFKV RALECHPDKH PENPKAVETF QKLQKAKEIL TNEESRARYD HWRRSQMSMP FQQWEALNDS VKTSMHWVVR GKKDLMLEES DKTHTTKMEN EECNEQRERK KEELASTAEK TEQKEPKPLE KSVSPQNSDS SGFADVNGWH LRFRWSKDAP SELLRKFRNY EI