Recombinant humaan DNAJC15-eiwit

DNAJC15 contains 1 J domain. This protein is expressed ubiquitously and located on the membrane. DNAJC15 is absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin. Recombinant human DNAJC15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DnaJ homolog subfamily C member 15, DNAJD1, HSD18, MCJ

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.8 kDa (116aa) confirmed by MALDI-TOF

DNAJC15, DnaJ homolog subfamily C member 15, DNAJD1, HSD18, MCJ, ATGP2300-10 µg, ATGP2300-20 µg, ATGP2300-50 µg, ATGP2300-100 µg, ATGP2300-250 µg, ATGP2300-500 µg, ATGP2300-1 mg, ATGP2300-10, ATGP2300-20, ATGP2300-50, ATGP2300-100, ATGP2300-250, ATGP2300-500, ATGP2300-1

Noskova L, Stranecky V, et al. (2011) . Am J Hum Genet. 89 (2) :241-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Cancer

NP_037370

Q9Y5T4

Human

MGSSHHHHHH SSGLVPRGSH MGSFRIWKPL EQVITETAKK ISTPSFSSYY KGGFEQKMSR REAGLILGVS PSAGKAKIRT AHRRVMILNH PDKGGSPYVA AKINEAKDLL ETTTKH