Recombinant humaan DNAJC24-eiwit

DnaJ homolog subfamily C member 24, also known as DNAJC24, is a member of the ZCSL family of proteins and each contain one DPH-type zinc finger. This enzyme is involved in the synthesis of diphthamide, a protein found on translation elongation factor EF-2 that is the target of bacterial ADP-ribosylating toxins. DNAJC24 stimulates the ATPase activity of several Hsp70-type chaperones. It is detected in heart, brain, spleen, lung, liver, kidney and testis. Recombinant human DNAJC24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DnaJ homolog subfamily C member 24, DPH4, JJJ3, ZCSL3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.5 kDa (172aa) confirmed by MALDI-TOF

DNAJC24, DnaJ homolog subfamily C member 24, DPH4, JJJ3, ZCSL3, ATGP2111-10 µg, ATGP2111-20 µg, ATGP2111-50 µg, ATGP2111-100 µg, ATGP2111-250 µg, ATGP2111-500 µg, ATGP2111-1 mg, ATGP2111-10, ATGP2111-20, ATGP2111-50, ATGP2111-100, ATGP2111-250, ATGP2111-500, ATGP2111-1

Liu S., et al. (2003) Mol Cell. 12:603-613; ; Liu S., et al. (2004) Mol Cell Biol. 244:9487-9497.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_859057

Q6P3W2

Human

MMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN