Recombinant humaan DNAL4-eiwit

DNAL4 is a component of the dynein motor complex in cells which converts the chemical energy contained in ATP into the mechanical energy of movement. Dynein transports various cellular cargo by 'walking' along cytoskeletal microtubules towards the minus-end of the microtubule, which is usually oriented towards the cell center. Recombinant human DNAL4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dynein light chain 4 axonemal, Dynein light chain 4, axonemal, PIG27

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.1 kDa (125aa) confirmed by MALDI-TOF

DNAL4, Dynein light chain 4 axonemal, Dynein light chain 4, axonemal, PIG27, ATGP1065-10 µg, ATGP1065-20 µg, ATGP1065-50 µg, ATGP1065-100 µg, ATGP1065-250 µg, ATGP1065-500 µg, ATGP1065-1 mg, ATGP1065-10, ATGP1065-20, ATGP1065-50, ATGP1065-100, ATGP1065-250, ATGP1065-500, ATGP1065-1

Rual JF. et al. (2005) Nature. 437: 1173-8.; ; Iwasaki M. et al. (2005) Blood. 105 : 784-93.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cytoskeleton

NP_005731

O96015

Human

MGETEGKKDE ADYKRLQTFP LVRHSDMPEE MRVETMELCV TACEKFSNNN ESAAKMIKET MDKKFGSSWH VVIGEGFGFE ITHEVKNLLY LYFGGTLAVC VWKCS