Recombinant humaan DNALI1-eiwit

DNALI1 is the human homolog of the Chlamydomonas inner dynein arm gene, p28. The precise function of this gene is not known, however, it is a potential candidate for immotile cilia yndrome (ICS) . ultrastructural defects of the inner dynein arms are seen in patients with ICS. Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. Recombinant human DNALI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Axonemal dynein light intermediate polypeptide 1, dJ423B22.5, hp28, P28

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.2 kDa (303aa) confirmed by MALDI-TOF

DNALI1, axonemal dynein light intermediate polypeptide 1, dJ423B22.5, hp28, P28, ATGP2410-10 µg, ATGP2410-20 µg, ATGP2410-50 µg, ATGP2410-100 µg, ATGP2410-250 µg, ATGP2410-500 µg, ATGP2410-1 mg, ATGP2410-10, ATGP2410-20, ATGP2410-50, ATGP2410-100, ATGP2410-250, ATGP2410-500, ATGP2410-1

Kastury K., et al (1997) . Biochem. J. Clin. Endocrinol. Metab. 82:3047-3053; ; Sjoeblom T., et al (2006) . Science 314:268-274 (2006) ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 0.1mM PMSF

Signal Transduction

NP_003453

O14645

Human

MGSSHHHHHH SSGLVPRGSH MGSMVTANKA HTGQGSCWVA TLASAMIPPA DSLLKYDTPV LVSRNTEKRS PKARLLKVSP QQPGPSGSAP QPPKTKLPST PCVPDPTKQA EEILNAILPP REWVEDTQLW IQQVSSTPST RMDVVHLQEQ LDLKLQQRQA RETGICPVRR ELYSQCFDEL IREVTINCAE RGLLLLRVRD EIRMTIAAYQ TLYESSVAFG MRKALQAEQG KSDMERKIAE LETEKRDLER QVNEQKAKCE ATEKRESERR QVEEKKHNEE IQFLKRTNQQ LKAQLEGIIA PKK