Recombinant humaan DNMT3L eiwit

DNA (cytosine-5) -methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA (cytosine-5) -methyltransferase 3-like isoform, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

46.3 kDa (412aa) confirmed by MALDI-TOF

DNMT3L, DNA (cytosine-5) -methyltransferase 3-like isoform, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like, ATGP2634-10 µg, ATGP2634-20 µg, ATGP2634-50 µg, ATGP2634-100 µg, ATGP2634-250 µg, ATGP2634-500 µg, ATGP2634-1 mg, ATGP2634-10, ATGP2634-20, ATGP2634-50, ATGP2634-100, ATGP2634-250, ATGP2634-500, ATGP2634-1

Yoder J A., et al. (1997) J Mol Biol. 270: 385-395.; ; Suetake I., et al. (2006) J Biochem 1405: 553-559.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Epigenomics (Transcription & Translation)

NP_037501

Q9UJW3

Human

MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSSRH WALVSEEELS LLAQNKQSSK LAAKWPTKLV KNCFLPLREY FKYFSTELTS SL