Recombinant humaan DNMT3L eiwit

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. DNMT3L is a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. It also mediates transcriptional repression through interaction with histone deacetylase 1. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

46.2 kDa (411aa) confirmed by MALDI-TOF

DNMT3L, DNA (cytosine-5) -methyltransferase 3-like isoform 2, DNA (cytosine-5-) -methyltransferase 3-like, ATGP2696-10 µg, ATGP2696-20 µg, ATGP2696-50 µg, ATGP2696-100 µg, ATGP2696-250 µg, ATGP2696-500 µg, ATGP2696-1 mg, ATGP2696-10, ATGP2696-20, ATGP2696-50, ATGP2696-100, ATGP2696-250, ATGP2696-500, ATGP2696-1

Ooi S.K., Qiu C, et al. (2007) Nature 448:714-717;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_787063

Q9UJW3

Human

MAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L