Recombinant humaan DNTTIP1-eiwit

DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template. Recombinant human DNTTIP1 fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Deoxynucleotidyltransferase terminal-interacting protein 1, C20orf167, dJ447F3.4, Tdif1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39 kDa (352aa) confirmed by MALDI-TOF

DNTTIP1, Deoxynucleotidyltransferase terminal-interacting protein 1, C20orf167, dJ447F3.4, Tdif1, ATGP2338-10 µg, ATGP2338-20 µg, ATGP2338-50 µg, ATGP2338-100 µg, ATGP2338-250 µg, ATGP2338-500 µg, ATGP2338-1 mg, ATGP2338-10, ATGP2338-20, ATGP2338-50, ATGP2338-100, ATGP2338-250, ATGP2338-500, ATGP2338-1

Yamashita N, Shimazaki N, et al. (2001) . Genes Cells. 6 (7) :641-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_443183

Q9H147

Human

MGSSHHHHHH SSGLVPRGSH MGSMGATGDA EQPRGPSGAE RGGLELGDAG AAGQLVLTNP WNIMIKHRQV QRRGRRSQMT TSFTDPAISM DLLRAVLQPS INEEIQTVFN KYMKFFQKAA LNVRDNVGEE VDAEQLIQEA CRSCLEQAKL LFSDGEKVIP RLTHELPGIK RGRQAEEECA HRGSPLPKKR KGRPPGHILS SDRAAAGMVW KPKSCEPIRR EGPKWDPARL NESTTFVLGS RANKALGMGG TRGRIYIKHP HLFKYAADPQ DKHWLAEQHH MRATGGKMAY LLIEEDIRDL AASDDYRGCL DLKLEELKSF VLPSWMVEKM RKYMETLRTE NEHRAVEAPP QT