Recombinant humaan DOK4-eiwit

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. This protein is putative link with downstream effectors of RET in neuronal differentiation. DOK4 may be involved in the regulation of the immune response induced by T-cells. Recombinant human DOK4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Docking protein 4, Downstream of tyrosine kinase 4, FLJ10488, IRS-5, IRS5

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.4 kDa (349aa)

DOK4, docking protein 4, Downstream of tyrosine kinase 4, FLJ10488, IRS-5, IRS5, ATGP2595-10 µg, ATGP2595-20 µg, ATGP2595-50 µg, ATGP2595-100 µg, ATGP2595-250 µg, ATGP2595-500 µg, ATGP2595-1 mg, ATGP2595-10, ATGP2595-20, ATGP2595-50, ATGP2595-100, ATGP2595-250, ATGP2595-500, ATGP2595-1

Hooker, E, et al. (2012) Biochem. Biophys. Res. Commun. 427 (1), 67-72; ; Gerard, A., et al. (2009) J. Immunol. 182 (12), 7681-7689;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Signal Transduction

NP_060580

Q8TEW6

Human

MGSSHHHHHH SSGLVPRGSH MGSMATNFSD IVKQGYVKMK SRKLGIYRRC WLVFRKSSSK GPQRLEKYPD EKSVCLRGCP KVTEISNVKC VTRLPKETKR QAVAIIFTDD SARTFTCDSE LEAEEWYKTL SVECLGSRLN DISLGEPDLL APGVQCEQTD RFNVFLLPCP NLDVYGECKL QITHENIYLW DIHNPRVKLV SWPLCSLRRY GRDATRFTFE AGRMCDAGEG LYTFQTQEGE QIYQRVHSAT LAIAEQHKRV LLEMEKNVRL LNKGTEHYSY PCTPTTMLPR SAYWHHITGS QNIAEASSYA GEGYGAAQAS SETDLLNRFI LLKPKPSQGD SSEAKTPSQ