Recombinant humaan Dopa Decarboxylase/DDC-eiwit

Dopa decarboxylase, also known as DDC, is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is a protein implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Dopa decarboxylase is found in different areas of the brain and is particularly abundant in basal ganglia. Recombinant human Dopa decarboxylase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

56.4 kDa (503aa) confirmed by MALDI-TOF

Dopa Decarboxylase, DDC, DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase, ATGP0267-10 µg, ATGP0267-20 µg, ATGP0267-50 µg, ATGP0267-100 µg, ATGP0267-250 µg, ATGP0267-500 µg, ATGP0267-1 mg, ATGP0267-10, ATGP0267-20, ATGP0267-50, ATGP0267-100, ATGP0267-250, ATGP0267-500, ATGP0267-1

Avgeris M., et al. (2008) . Clin Biochem. 41 (14-15) :1140-9.; ; Pons R., et al. (2004) . Neruology. 62 (7) :1058-65.;

Uenishi E, et al. A novel mechanism of idiopathic orthostatic hypotension and hypocatecholaminemia due to autoimmunity against aromatic l-Amino acid decarboxylase. (Biochem Biophys Res Commun. 2024) {https://pubmed.ncbi.nlm.nih.gov/38677008/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol

Neuroscience

NP_001076440.2

P20711

Human

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