Recombinant humaan Dopamine beta-Hydroxylase-eiwit

DBH also known as dopamine beta hydroxylase that catalyzes the chemical reaction. DBH belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one ato of oxygen into the other donor. Recombinant human DBH, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dopamine beta hydroxylase, Dopamine beta hydroxylase, DBM

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

67.2 kDa (599aa)

Dopamine b-Hydroxylase, Dopamine beta hydroxylase, Dopamine beta hydroxylase, DBM, ATGP3032-10 µg, ATGP3032-20 µg, ATGP3032-50 µg, ATGP3032-100 µg, ATGP3032-250 µg, ATGP3032-500 µg, ATGP3032-1 mg, ATGP3032-10, ATGP3032-20, ATGP3032-50, ATGP3032-100, ATGP3032-250, ATGP3032-500, ATGP3032-1

Rush RA., et al. (1980) Crit Rev Clin Lab Sci. 12 (3) : 241-77

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Neuroscience

NP_000778

P09172

Human

SAPRESPLP YHIPLDPEGS LELSWNVSYT QEAIHFQLLV RRLKAGVLFG MSDRGELENA DLVVLWTDGD TAYFADAWSD QKGQIHLDPQ QDYQLLQVQR TPEGLTLLFK RPFGTCDPKD YLIEDGTVHL VYGILEEPFR SLEAINGSGL QMGLQRVQLL KPNIPEPELP SDACTMEVQA PNIQIPSQET TYWCYIKELP KGFSRHHIIK YEPIVTKGNE ALVHHMEVFQ CAPEMDSVPH FSGPCDSKMK PDRLNYCRHV LAAWALGAKA FYYPEEAGLA FGGPGSSRYL RLEVHYHNPL VIEGRNDSSG IRLYYTAKLR RFNAGIMELG LVYTPVMAIP PRETAFILTG YCTDKCTQLA LPPSGIHIFA SQLHTHLTGR KVVTVLVRDG REWEIVNQDN HYSPHFQEIR MLKKVVSVHP GDVLITSCTY NTEDRELATV GGFGILEEMC VNYVHYYPQT QLELCKSAVD AGFLQKYFHL INRFNNEDVC TCPQASVSQQ FTSVPWNSFN RDVLKALYSF APISMHCNKS SAVRFQGEWN LQPLPKVIST LEEPTPQCPT SQGRSPAGPT VVSIGGGKG