Recombinant humaan DPH2-eiwit

DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE) . It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Diphthamide biosynthesis protein 2, DPH2, DPH2L2

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

53.1 kDa (497aa)

DPH2, Diphthamide biosynthesis protein 2, DPH2, DPH2L2, ATGP3582-10 µg, ATGP3582-20 µg, ATGP3582-50 µg, ATGP3582-100 µg, ATGP3582-250 µg, ATGP3582-500 µg, ATGP3582-1 mg, ATGP3582-10, ATGP3582-20, ATGP3582-50, ATGP3582-100, ATGP3582-250, ATGP3582-500, ATGP3582-1

Zhang Y., et al. (2010) Nature. 465:891-896.; ; Mattheakis LC., et al. (1993) Gene. 132:149-154.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol

Cell Cycle

NP_001375

Q9BQC3

Human

Met1