Recombinant humaan DPPA4-eiwit

DPPA4 may be involved in the maintenance of active epigenetic status of target genes and may inhibit differentiation of embryonic cells into a primitive ectoderm lineage. Recombinant human DPPA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Developmental pluripotency associated 4, 2410091M23Rik

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.9 kDa (327aa) confirmed by MALDI-TOF

DPPA4, Developmental pluripotency associated 4, 2410091M23Rik, ATGP2199-10 µg, ATGP2199-20 µg, ATGP2199-50 µg, ATGP2199-100 µg, ATGP2199-250 µg, ATGP2199-500 µg, ATGP2199-1 mg, ATGP2199-10, ATGP2199-20, ATGP2199-50, ATGP2199-100, ATGP2199-250, ATGP2199-500, ATGP2199-1

Ota T., et al. (2004) Nat. Genet. 36:40-45; ; Muzny D.M., et al. (2006) Nature. 440:1194-1198;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Development

NP_060659.3

Q7L190

Human

MLRGSAS STSMEKAKGK EWTSTEKSRE EDQQASNQPN SIALPGTSAK RTKEKMSIKG SKVLCPKKKA EHTDNPRPQK KIPIPPLPSK LPPVNLIHRD ILRAWCQQLK LSSKGQKLDA YKRLCAFAYP NQKDFPSTAK EAKIRKSLQK KLKVEKGETS LQSSETHPPE VALPPVGEPP ALENSTALLE GVNTVVVTTS APEALLASWA RISARARTPE AVESPQEASG VRWCVVHGKS LPADTDGWVH LQFHAGQAWV PEKQEGRVSA LFLLPASNFP PPHLEDNMLC PKCVHRNKVL IKSLQWE