Recombinant humaan DPY30-eiwit

As part of the MLL1/MLL complex, DPY30 is involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. This protein may play some role in histone H3 acetylation. In a teratocarcinoma cell, it plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. DPY30 may also play an indirect or direct role in endosomal transport. Recombinant human DPY30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein dpy-30 homolog, HDPY-30, Saf19

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

13.6 kDa (122aa) confirmed by MALDI-TOF

DPY30, protein dpy-30 homolog, HDPY-30, Saf19, ATGP2797-10 µg, ATGP2797-20 µg, ATGP2797-50 µg, ATGP2797-100 µg, ATGP2797-250 µg, ATGP2797-500 µg, ATGP2797-1 mg, ATGP2797-10, ATGP2797-20, ATGP2797-50, ATGP2797-100, ATGP2797-250, ATGP2797-500, ATGP2797-1

Xu Z., et al (2009) . J. Cell Biol. 186:343-353; ; Patel A., et al (2009) . J. Biol. Chem. 284:24242-24256

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT

Other

NP_115963

Q9C005

Human

MEPEQML EGQTQVAENP HSEYGLTDNV ERIVENEKIN AEKSSKQKVD LQSLPTRAYL DQTVVPILLQ GLAVLAKERP PNPIEFLASY LLKNKAQFED RN