Recombinant humaan DSTN-eiwit

Destrin (actin depolymerizing factor), also known as DSTN, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. DSTN is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. DSTN shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. Recombinant human DSTN protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Destrin (actin depolymerizing factor), ACTDP, ADF, bA462D18.2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19.5 kDa (173aa) confirmed by MALDI-TOF

DSTN, Destrin (actin depolymerizing factor), ACTDP, ADF, bA462D18.2, ATGP1596-10 µg, ATGP1596-20 µg, ATGP1596-50 µg, ATGP1596-100 µg, ATGP1596-250 µg, ATGP1596-500 µg, ATGP1596-1 mg, ATGP1596-10, ATGP1596-20, ATGP1596-50, ATGP1596-100, ATGP1596-250, ATGP1596-500, ATGP1596-1

Nishida E., et al. (1985) Biochemistry. 24:6624-6630.; ; Kanamori T., et al. (1995) J Biol Chem. 270: 8061-8067.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Actin & Myosin Related

NP_006861

P60981

Human

MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH