Recombinant humaan DTD1-eiwit

DTD1 hydrolyzes D-tyrosyl-tRNA (Tyr) into D-tyrosine and free tRNA (Tyr) . It could be a defense mechanism against a harmful effect of D-tyrosine. DTD1 may be a risk factor for aspirin-intolerant asthma (AIA) by catalyzing the hydrolysis of D-tryptophan and interacting with the tyrosyl-tRNA synthetase (tyrRS) enzyme, which promotes a pro-inflammatory phenotype. This protein is localize to the DuE (DNA unwinding element) s of active replication origins. Recombinant human DTD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

D-tyrosyl-tRNA deacylase 1, bA379J5.3, bA555E18.1, C20orf88, DuEB, HARS2, pqn-68

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.9 kDa (232aa) confirmed by MALDI-TOF

DTD1, D-tyrosyl-tRNA deacylase 1, bA379J5.3, bA555E18.1, C20orf88, DuEB, HARS2, pqn-68, ATGP1831-10 µg, ATGP1831-20 µg, ATGP1831-50 µg, ATGP1831-100 µg, ATGP1831-250 µg, ATGP1831-500 µg, ATGP1831-1 mg, ATGP1831-10, ATGP1831-20, ATGP1831-50, ATGP1831-100, ATGP1831-250, ATGP1831-500, ATGP1831-1

Pasaje CF, et al. (2011) Int J Mol Med. 28 (1) :129-37. ; ; Chowdhury A, et al. (2010) Mol Cell Biol. 30 (6) :1495-507.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_543010

Q8TEA8

Human

MGSSHHHHHH SSGLVPRGSH MGSMKAVVQR VTRASVTVGG EQISAIGRGI CVLLGISLED TQKELEHMVR KILNLRVFED ESGKHWSKSV MDKQYEILCV SQFTLQCVLK GNKPDFHLAM PTEQAEGFYN SFLEQLRKTY RPELIKDGKF GAYMQVHIQN DGPVTIELES PAPGTATSDP KQLSKLEKQQ QRKEKTRAKG PSESSKERNT PRKEDRSASS GAEGDVSSER EP