Recombinant humaan DTNBP1-eiwit

Dysbindin is a ubiquitously expressed protein that binds to alpha- and beta-dystrobrevins, components of the dystrophin-associated protein complex (DPC) in both muscle and nonmuscle cells. Dysbindin has been strongly implicated in schizophrenia (SZ) susceptibility by a series of independent genetic association and gene expression studies. Among its known functions, dysbindin is part of a protein complex, termed the biogenesis of lysosome-related organelles complex 1 (BLOC-1), the molecular components of which might be involved in the regulation of vesicular trafficking and dendrite branching. Dysbindin effects for brain volume and cortical thickness appear driven by different neurobiological processes. Recombinant human Dysbindin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dystrobrevin-binding protein 1 isoform C, DTNBP1, DBND, HPS7, SDY, Dystrobrevin-binding protein 1 isoform C Dystrobrevin binding protein 1, Hermansky Pudlak syndrome 7 protein, Dysbindin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.5 kDa (306aa) confirmed by MALDI-TOF

Dysbindin, Dystrobrevin-binding protein 1 isoform C, DTNBP1, DBND, HPS7, SDY, Dystrobrevin-binding protein 1 isoform C Dystrobrevin binding protein 1, Hermansky Pudlak syndrome 7 protein., ATGP0278-10 µg, ATGP0278-20 µg, ATGP0278-50 µg, ATGP0278-100 µg, ATGP0278-250 µg, ATGP0278-500 µg, ATGP0278-1 mg, ATGP0278-10, ATGP0278-20, ATGP0278-50, ATGP0278-100, ATGP0278-250, ATGP0278-500, ATGP0278-1

Li W., et al. (2003) . Nat Genet. 35 (1) :84-9; ; Chen XW., et al. (2008) . J Cell Biol. 181 (5) :791-801;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.5mM DTT, 20% glycerol

Neuroscience

NP_898862.1

Q96EV8

Human

MLSA HWEKKKTSLV ELQEQLQQLP ALIADLESMT ANLTHLEASF EEVENNLLHL EDLCGQCELE RCKHMQSQQ LENYKKNKRKE LETFKAELDA EHAQKVLEME HTQQMKLKER QKFFEEAFQQ DMEQYLSTGY LQIAERREPI GSMSSMEVNV DMLEQMDLMD ISDQEALDVF LNSGGEENTV LSPALGPESS TCQNEITLQV PNPSELRAKP PSSSSTCTDS ATRDISEGGE SPVVQSDEEE VQVDTALATS HTDREATPDG GEDSDS