Recombinant humaan DTYMK-eiwit

DTYMK is also known as thymidylate kinase family and is involved in pyrimidine metabolism. Specifically, DTYMK catalyzes the ATP-dependent conversion of dTMP (deoxythymidine monophosphate) to dTDP (deoxythymidine diphosphate), which then functions as one of the four nucleotides in DNA. Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth. Recombinant human DTYMK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Thymidylate kinase, CDC8, FLJ44192, MGC198617, PP3731, TMPK, TYMK

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26 kDa (232aa) confirmed by MALDI-TOF

DTYMK, Thymidylate kinase, CDC8, FLJ44192, MGC198617, PP3731, TMPK, TYMK, ATGP1114-10 µg, ATGP1114-20 µg, ATGP1114-50 µg, ATGP1114-100 µg, ATGP1114-250 µg, ATGP1114-500 µg, ATGP1114-1 mg, ATGP1114-10, ATGP1114-20, ATGP1114-50, ATGP1114-100, ATGP1114-250, ATGP1114-500, ATGP1114-1

Cummins RR., et al. (1980) Cancer Res. 40 (4) :1235-9.; ; Su JY., et al. (1991) Nucleic Acids Res. 19 (4) :823-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl

Cell Cycle

NP_036277.2

P23919

Human

MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK