Recombinant humaan DUSP10-eiwit

DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Dual specificity protein phosphatase 10, MKP-5, MKP5, Mitogen-activated protein kinase phosphatase 5, MAP kinase phosphatase 5

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 600unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

40.4 kDa (359aa) confirmed by MALDI-TOF

DUSP10, Dual specificity protein phosphatase 10, MKP-5, MKP5, ATGP1392-10 µg, ATGP1392-20 µg, ATGP1392-50 µg, ATGP1392-100 µg, ATGP1392-250 µg, ATGP1392-500 µg, ATGP1392-1 mg, ATGP1392-10, ATGP1392-20, ATGP1392-50, ATGP1392-100, ATGP1392-250, ATGP1392-500, ATGP1392-1

Theodosiou A, et al. (1999) Oncogene. 18 (50) :6981-8.; ; Jeong DG, et al. (2006) J Mol Biol. 360 (5) :946-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200 mM NaCl

Enzymes & Proteases

NP_009138

Q9Y6W6

Human

IIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV