Recombinant humaan DUSP21-eiwit

DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dual specificity protein phosphatase 21, Low molecular weight dual specificity phosphatase 21, LMW-DSP21

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.1 kDa (214aa) confirmed by MALDI-TOF

DUSP21, Dual specificity protein phosphatase 21, ATGP1393-10 µg, ATGP1393-20 µg, ATGP1393-50 µg, ATGP1393-100 µg, ATGP1393-250 µg, ATGP1393-500 µg, ATGP1393-1 mg, ATGP1393-10, ATGP1393-20, ATGP1393-50, ATGP1393-100, ATGP1393-250, ATGP1393-500, ATGP1393-1

Hood, K.L., et al. (2002) Biochem. Biophys. Res. Commun. 298: 545-551.; ; Keyse, S.M. (1995) Biochim. Biophys. Acta 1265: 152-160.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Enzymes & Proteases

NP_071359

Q9H596

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM