Recombinant humaan DUTPase/DUT-eiwit

DuT, also known as duTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing duTP to duMP and diphosphate. This reaction is important for keeping the intracellular duTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DuT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DuT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

FLJ20622, duTPase, duTP pyrophosphatase, duTP diphosphatase, Deoxyuridine Triphosphatase, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.6 kDa (204aa) confirmed by MALDI-TOF

DuT, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, duTPase, duTP pyrophosphatase, FLJ20622, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, Deoxyuridine Triphosphatase, ATGP0725-10 µg, ATGP0725-20 µg, ATGP0725-50 µg, ATGP0725-100 µg, ATGP0725-250 µg, ATGP0725-500 µg, ATGP0725-1 mg, ATGP0725-10, ATGP0725-20, ATGP0725-50, ATGP0725-100, ATGP0725-250, ATGP0725-500, ATGP0725-1

Mclntosh EM., et al. (1992) Proc Natl Acad Sci u S A. 89 (17) :8020-4. ; ; Persson R., et al. (2001) Curr Protein Pept Sci. 2 (4) :287-300.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Enzymes & Proteases

NP_001020419

P33316

Human

MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN