Recombinant humaan Dynactin Subunit 2/DCTN2-eiwit

Dynactin 2, also known as DCTN2, modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. This protein is involved in anchoring microtubules to centrosomes. This protein may play a role in synapse formation during brain development. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dynactin 2 isoform 2, dynactin 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

46.9 kDa (426aa) confirmed by MALDI-TOF

Dynactin Subunit2, DCTN2, Dynactin 2 isoform 2, dynactin 2, ATGP2450-10 µg, ATGP2450-20 µg, ATGP2450-50 µg, ATGP2450-100 µg, ATGP2450-250 µg, ATGP2450-500 µg, ATGP2450-1 mg, ATGP2450-10, ATGP2450-20, ATGP2450-50, ATGP2450-100, ATGP2450-250, ATGP2450-500, ATGP2450-1

Echeverri C.J., et al. (1996) J. Cell Biol. 132:617-633; ; Gevaert K., et al. (2003) Nat. Biotechnol. 21:566-569

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_001248341

Q13561

Human

MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEL EELTSTSVEH IIVNPNAAYD KFKDKRVGTK GLDFSDRIGK TKRTGYESGE YEMLGEGLGV KETPQQKYQR LLHEVQELTT EVEKIKTTVK ESATEEKLTP VLLAKQLAAL KQQLVASHLE KLLGPDAAIN LTDPDGALAK RLLLQLEATK NSKGGSGGKT TGTPPDSSLV TYELHSRPEQ DKFSQAAKVA ELEKRLTELE TAVRCDQDAQ NPLSAGLQGA CLMETVELLQ AKVSALDLAV LDQVEARLQS VLGKVNEIAK HKASVEDADT QSKVHQLYET IQRWSPIAST LPELVQRLVT IKQLHEQAMQ FGQLLTHLDT TQQMIANSLK DNTTLLTQVQ TTMRENLATV EGNFASIDER MKKLGK