Recombinant humaan Dynactin Subunit 2/DCTN2-eiwit

DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dynactin subunit 2 isoform 1, Dynactin subunit 2 isoform 1, Dynactin 2 (p50), DCTN50, DYNAMITIN, RBP50

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.2 kDa (429aa) confirmed by MALDI-TOF

Dynactin Subunit2, DCTN2, Dynactin subunit 2 isoform 1, Dynactin subunit 2 isoform 1, Dynactin 2 (p50), DCTN50, DYNAMITIN, RBP50, ATGP2490-10 µg, ATGP2490-20 µg, ATGP2490-50 µg, ATGP2490-100 µg, ATGP2490-250 µg, ATGP2490-500 µg, ATGP2490-1 mg, ATGP2490-10, ATGP2490-20, ATGP2490-50, ATGP2490-100, ATGP2490-250, ATGP2490-500, ATGP2490-1

Echeverri C.J., Paschal B.M. et al. (1996), J. Cell Biol. 132:617-633

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_006391.1

Q13561

Human

MADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAFA QELEELTSTS VEHIIVNPNA AYDKFKDKRV GTKGLDFSDR IGKTKRTGYE SGEYEMLGEG LGVKETPQQK YQRLLHEVQE LTTEVEKIKT TVKESATEEK LTPVLLAKQL AALKQQLVAS HLEKLLGPDA AINLTDPDGA LAKRLLLQLE ATKNSKGGSG GKTTGTPPDS SLVTYELHSR PEQDKFSQAA KVAELEKRLT ELETAVRCDQ DAQNPLSAGL QGACLMETVE LLQAKVSALD LAVLDQVEAR LQSVLGKVNE IAKHKASVED ADTQSKVHQL YETIQRWSPI ASTLPELVQR LVTIKQLHEQ AMQFGQLLTH LDTTQQMIAN SLKDNTTLLT QVQTTMRENL ATVEGNFASI DERMKKLGK