Recombinant humaan DYNLL2-eiwit

Dynein light chain 2, cytoplasmic, also known DYNLL2, is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. DYNLL2 is a highly conserved eukaryotic hub protein with dozens of binding partners and various functions beyond being a subunit of dynein and myosin Va motor proteins. Recombinant human DYNLL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dynein light chain 2 LC8-type 2, Dynein light chain 2, LC8-type 2, Dlc2, DNCL1B, DLC8b

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.5 kDa (109aa) confirmed by MALDI-TOF

DYNLL2, Dynein light chain 2 LC8-type 2, Dynein light chain 2, LC8-type 2, Dlc2, DNCL1B, DLC8b, ATGP1087-10 µg, ATGP1087-20 µg, ATGP1087-50 µg, ATGP1087-100 µg, ATGP1087-250 µg, ATGP1087-500 µg, ATGP1087-1 mg, ATGP1087-10, ATGP1087-20, ATGP1087-50, ATGP1087-100, ATGP1087-250, ATGP1087-500, ATGP1087-1

Radnai L., et al. (2010) J Biol chem. 285 (49) :38649-57.; ; Lu J., et al. (2005) Biochem Biophys Res Commun. 331 (1) :153-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Cytoskeleton

NP_542408

Q96FJ2

Human

MGSSHHHHHH SSGLVPRGSH MSDRKAVIKN ADMSEDMQQD AVDCATQAME KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG