Recombinant humaan E-selectine/CD62E-eiwit

E-selectin, also known as CD62 antigen-like family member E (CD62E), is a member of the Selectin family. It is a selectin cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. The local release of cytokines IL-1 and TNF-α by Macrophages in the inflamed tissue induces the over-expression of E-selectin on endothelial cells of nearby blood vessels. As the inflammatory response progresses, chemokines released by injured tissue enter the blood vessels and activate the rolling leukocytes, which are now able to tightly bind to the endothelial surface and begin making their way into the tissue. While numerous pieces of in vitro and clinical evidence continue to support this hypothesis of E-selectin-mediated cancer metastasis, in vivo studies of cancer metastasis have shown that E-selectin knockout only minimally affects leukemic cell adhesion to bone immediately following injection. Recombinant human E-selectin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human E-Seletin/CD62E stimulates cell adhesion of the U937 human histiocytic lymphoma cells. This effect is more to 40%.

E-selectin, CD62E, ELAM, ELAM1, ELAM-1, ESEL, LECAM2, CD62 antigen-like family member E, endothelial-leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, SELE, CD62E antigen, ELAM1E-selectin, Endothelial leukocyte adhesion molecule 1

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human E-Seletin/CD62E coated plates 2 µg/mL. This effect is more to 40%.

59.4kDa (541aa)

, ATGP3994-10 µg, ATGP3994-20 µg, ATGP3994-50 µg, ATGP3994-100 µg, ATGP3994-250 µg, ATGP3994-500 µg, ATGP3994-1 mg, ATGP3994-10, ATGP3994-20, ATGP3994-50, ATGP3994-100, ATGP3994-250, ATGP3994-500, ATGP3994-1

Collins T, et al., (1991), J. Biol. Chem. 266:2466–2473.; ; Kawase J, et al. (2009) . Oncol Rep. 22:1293-1297.; ; Matsumoto K, et al. (2010) . Metabolism. 59:320-324.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology, Adhesion & Movement

NP_000441

P16581

Human

Trp 22