Recombinant humaan E2-EFF/UBE2S-eiwit

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins. Recombinant uBE2S protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.9 kDa (258aa) confirmed by MALDI-TOF

UBE2S, ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2S AA409170, MGC101982, uBE2S, ubiquitin carrier protein S, ubiquitin protein ligase S, ubiquitin conjugating enzyme E2 24 kD, ubiquitin conjugating enzyme E2S, ATGP0372-10 µg, ATGP0372-20 µg, ATGP0372-50 µg, ATGP0372-100 µg, ATGP0372-250 µg, ATGP0372-500 µg, ATGP0372-1 mg, ATGP0372-10, ATGP0372-20, ATGP0372-50, ATGP0372-100, ATGP0372-250, ATGP0372-500, ATGP0372-1

Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10 (11) :755-64. ; ; Windheim M., et al. (2008) Biochem J. 409 (3) :723-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT and 20% glycerol

Ubiquitination

NP_055316.2

Q16763

Human

MNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL