Recombinant humaan ECH1-eiwit

ECH1 (enoyl coenzyme A hydratase 1) belongs to the hydratase/isomerase superfamily. This Protein shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. It contains a C-terminal peroxisomal targeting sequence, localizes to both the peroxisome and the mitochondria. This enzyme involved in the auxiliary step of the fatty acid beta-oxidation pathway specifically functioning to catalyze the isomerization of 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-transdienoyl-CoA. Recombinant human ECH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Enoyl Coenzyme A hydratase 1 peroxisomal, enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.4 kDa (316aa) confirmed by MALDI-TOF

ECH1, enoyl Coenzyme A hydratase 1 peroxisomal, enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL, ATGP0711-10 µg, ATGP0711-20 µg, ATGP0711-50 µg, ATGP0711-100 µg, ATGP0711-250 µg, ATGP0711-500 µg, ATGP0711-1 mg, ATGP0711-10, ATGP0711-20, ATGP0711-50, ATGP0711-100, ATGP0711-250, ATGP0711-500, ATGP0711-1

Goehler H., et al. (2004) Mol. Cell 15 (6) :853-65.; ; FitzPatrick DR., et al. (1995) Genomics 27 (3) :457-66.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Metabolism

AAH17408

Q13011

Human

TGSSAQEAA SGVALGEAPD HSYESLRVTS AQKHVLHVQL NRPNKRNAMN KVFWREMVEC FNKISRDADC RAVVISGAGK MFTAGIDLMD MASDILQPKG DDVARISWYL RDIITRYQET FNVIERCPKP VIAAVHGGCI GGGVDLVTAC DIRYCAQDAF FQVKEVDVGL AADVGTLQRL PKVIGNQSLV NELAFTARKM MADEALGSGL VSRVFPDKEV MLDAALALAA EISSKSPVAV QSTKVNLLYS RDHSVAESLN YVASWNMSML QTQDLVKSVQ ATTENKELKT VTFSKL