Recombinant humaan ECHS1-eiwit

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.6 kDa (284aa) confirmed by MALDI-TOF

ECHS1, Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, ATGP0703-10 µg, ATGP0703-20 µg, ATGP0703-50 µg, ATGP0703-100 µg, ATGP0703-250 µg, ATGP0703-500 µg, ATGP0703-1 mg, ATGP0703-10, ATGP0703-20, ATGP0703-50, ATGP0703-100, ATGP0703-250, ATGP0703-500, ATGP0703-1

Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13; ; Stern J R., et al. (1956) J Biol Chem. 218:985-1002.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Metabolism

AAH08906

P30084

Human

MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ