Recombinant humaan ECSIT-eiwit

ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF) -6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.6 kDa (222aa) confirmed by MALDI-TOF

ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC, ATGP1745-10 µg, ATGP1745-20 µg, ATGP1745-50 µg, ATGP1745-100 µg, ATGP1745-250 µg, ATGP1745-500 µg, ATGP1745-1 mg, ATGP1745-10, ATGP1745-20, ATGP1745-50, ATGP1745-100, ATGP1745-250, ATGP1745-500, ATGP1745-1

Moustakas A., et al. (2003) Genes Dev17:2855-2859; ; Vogel R O., et al. (2007) Genes Dev. 21: 615-624.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Immunology

NP_001135937

Q9BQ95

Human

MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS