Recombinant humaan EDAR-eiwit

EDAR, also known as tumor necrosis factor receptor superfamily member EDAR, is a single-pass type 1 transmembrane protein which is a member of TNF receptor superfamily. This protein was expressed reiteratively in signaling centers regulating key steps in morphogenesis. Also, it is a cell surface receptor for ectodysplasin A which plays an important role in the development of ectodermal tissues such as the skin. Also, defects in EDAR are a cause of ectodermal dysplasia anhidrotic (EDA), also known ectodermal dysplasia hypohidrotic autosomal recessive (HED) . Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structure. Recombinant human EDAR, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tumor necrosis factor receptor superfamily member EDAR, EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

45.6 kDa (413aa)

EDAR, Tumor necrosis factor receptor superfamily member EDAR, EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1, ATGP3759-10 µg, ATGP3759-20 µg, ATGP3759-50 µg, ATGP3759-100 µg, ATGP3759-250 µg, ATGP3759-500 µg, ATGP3759-1 mg, ATGP3759-10, ATGP3759-20, ATGP3759-50, ATGP3759-100, ATGP3759-250, ATGP3759-500, ATGP3759-1

Kumar A., et al., (2000) J. Biol. Chem. 276:2668-2677.; ; Schneider P, et al., (2001) J. Biol. Chem. 276:18819-18827.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_071731

Q9UNE0

Human

Ala

ADPEYSNCGE NEYYNQTTGL CQECPPCGPG EEPYLSCGYG TKDEDYGCVP CPAEKFSKGG YQICRRHKDC EGFFRATVLT PGDMENDAEC GPCLPGYYML ENRPRNIYGM VCYSCLLAPP NTKECVGATS GASANFPGTS GSSTLSPFQH AHKELSGQGH LATAAAAFES RACSLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH