Recombinant humaan EED-eiwit

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta 7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Recombinant EED protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Polycomb protein EED isoform a, embryonic ectoderm development, HEED; WAIT1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

52.6 kDa (464aa)

EED, ATGP2154-10 µg, ATGP2154-20 µg, ATGP2154-50 µg, ATGP2154-100 µg, ATGP2154-250 µg, ATGP2154-500 µg, ATGP2154-1 mg, ATGP2154-10, ATGP2154-20, ATGP2154-50, ATGP2154-100, ATGP2154-250, ATGP2154-500, ATGP2154-1

Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998) . J Biol Chem 273 (42) : 27459-66; ; Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999) . Genomics 54 (1) : 79-88

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_003788.2

O75530

Human

MSEREVS TAPAGTDMPA AKKQKLSSDE NSNPDLSGDE NDDAVSIESG TNTERPDTPT NTPNAPGRKS WGKGKWKSKK CKYSFKCVNS LKEDHNQPLF GVQFNWHSKE GDPLVFATVG SNRVTLYECH SQGEIRLLQS YVDADADENF YTCAWTYDSN TSHPLLAVAG SRGIIRIINP ITMQCIKHYV GHGNAINELK FHPRDPNLLL SVSKDHALRL WNIQTDTLVA IFGGVEGHRD EVLSADYDLL GEKIMSCGMD HSLKLWRINS KRMMNAIKES YDYNPNKTNR PFISQKIHFP DFSTRDIHRN YVDCVRWLGD LILSKSCENA IVCWKPGKME DDIDKIKPSE SNVTILGRFD YSQCDIWYMR FSMDFWQKML ALGNQVGKLY VWDLEVEDPH KAKCTTLTHH KCGAAIRQTS FSRDSSILIA VCDDASIWRW DRLR