Recombinant humaan EEF2-eiwit

EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Elongation factor 2, EEF-2, EF-2, EF2, Eukaryotic translation elongation factor 2, Polypeptidyl-tRNA translocase

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.3 kDa (308aa)

EEF2, elongation factor 2, EEF-2, EF-2, EF2, ATGP2764-10 µg, ATGP2764-20 µg, ATGP2764-50 µg, ATGP2764-100 µg, ATGP2764-250 µg, ATGP2764-500 µg, ATGP2764-1 mg, ATGP2764-10, ATGP2764-20, ATGP2764-50, ATGP2764-100, ATGP2764-250, ATGP2764-500, ATGP2764-1

Yamashita A., et al (2009) . Genes Dev. 23:1091-1105; ; Olsen J.V., et al (2006) . Cell 127:635-648

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_001952.1

P13639

Human

DPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL