Recombinant humaan EF-1 alfa1/EEF1A1-eiwit

EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Recombinant human EEF1A1 protein is expressed in E. coli.

Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1

E.coli

Human

Non-Tagged

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50.1 kDa (462aa)

EEF1A1, Eukaryotic translation elongation factor 1 alpha 1, CCS-3, CCS3, EEF-1, EEF1A, eEF1A-1, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, PTI1, ATGP2613-10 µg, ATGP2613-20 µg, ATGP2613-50 µg, ATGP2613-100 µg, ATGP2613-250 µg, ATGP2613-500 µg, ATGP2613-1 mg, ATGP2613-10, ATGP2613-20, ATGP2613-50, ATGP2613-100, ATGP2613-250, ATGP2613-500, ATGP2613-1

Meriin AB, Zaarur N, et al. (2012) . J Cell Sci. 125 (Pt 11) :2665-74. ; ; Itagaki K, Naito T, et al. (2012) . J Biol Chem. 287 (19) :16037-46. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_001393.1

P68104

Human

MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK