Recombinant humaan EF-1 bèta/EEF1B2-eiwit

Elongation factor 1-beta, also known as EEF1B2, is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. EEF1B2 and EEF1D stimulate the exchange of GDP bound to EEF1A to GTP. Recombinant human EEF1B2 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Elongation factor 1-beta, EF-1-beta, EEF1B, EEF1B1, EF1B, Eukaryotic translation elongation factor 1 beta 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.8 kDa (233aa) confirmed by MALDI-TOF

EEF1B2, Elongation factor 1-beta, EF-1-beta, EEF1B, EEF1B1, EF1B, ATGP0946-10 µg, ATGP0946-20 µg, ATGP0946-50 µg, ATGP0946-100 µg, ATGP0946-250 µg, ATGP0946-500 µg, ATGP0946-1 mg, ATGP0946-10, ATGP0946-20, ATGP0946-50, ATGP0946-100, ATGP0946-250, ATGP0946-500, ATGP0946-1

Byun HO., et al. (2009) Cancer Res, 69 (11) :4638-47.; ; Sang Lee J., et al. (2002) Biochem Biophys Res Commun. 291 (1) :158-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Metabolism

NP_066944

P24534

Human